O-073 Delta-9-THC acts on the calcium channel CatSper and alters human sperm function

Abstract Study question Do the main psychoactive phytocannabinoid delta-9-tetrahydrocannabinol (THC) and its non-psychoactive analog cannabidiol (CBD) affect human sperm function? Summary answer THC reduces ligand-dependent activation of sperm-specific Ca2+ channel CatSper affects functions in vitro. What is known already Marijuana (Cannabis sativa) one most commonly used recreational drugs worldwide. Although impact phytocannabinoids on reproductive health has been investigated, there no evidence a direct effect function. design, size, duration We studied effects phytocannabinoid, THC, analog, CBD, as well their major metabolites influx via spermatozoa. CBD were selected to further evaluate action progesterone-, prostaglandin-, pH-induced CatSper. The hyperactivation, progressive motility viscous media, acrosomal exocytosis also assessed. Participants/materials, setting, methods activity was investigated samples from healthy volunteers using kinetic fluorimetry. Motility assessment performed Computer-Assisted Sperm Analysis (CASA). penetration into media assessed modified Kremer test. Acrosomal evaluated by flow cytometry Pisum sativum agglutinin-stained Main results role chance Both suppress natural ligand-induced calcium In particular, inhibits progesterone-induced at pharmacologically relevant concentrations non-competitive manner addition, impaired hyperactivation induced spontaneous Limitations, reasons for caution This an vitro study. Future studies are needed test physiological relevance vivo whether can disrupt Wider implications findings might impair fertilization process. Healthcare providers, especially fertility clinicians, should be aware potentially negative cannabis consumption physiology Trial registration number not applicable